ANALYSIS OF THE EFFECT OF GINGER ON REDUCING COX-2 GENE EXPRESSION IN “HT-29” COLON CANCER STEM CELLS

 

Haghighi, A.1, Zia Jahromi, N.2, Hejazi, S.H.3

1-       MSc. In Biochemistry, School of Basic Sciences, Islamic Azad University/Shahrekord Branch

2-       Assistant Professor in Biology group, School of Basic Sciences, Islamic Azad University/Shahrekord Branch

3-       Professor in the Sedigheh Tahereh Skin Diseases and Leishmaniasis Center, Parasitology and Mycology Group, School of Medicine, Esfahan University of Medical Sciences

 * Correspondance: This email address is being protected from spambots. You need JavaScript enabled to view it. (+98933146504)

 

ABSTRACT:

Introduction and objective:Colon cancer is the second reason of mortality caused by cancer in the world, after lung cancer, and it is the third prevalent gastrointestinal cancer after gastric and esophageal cancers in Iran. Environmental and genetic factors have important roles in the emergence of this cancer. One of the important factors in creation of this type of cancer is chronic inflammations of colon. Ginger has anti-inflammatory properties due to its content of [6]-gingerol and, hence, it can play a role in the prevention of colon cancer. COX-2 gene expression is increased in colon cancer, causing intestinal inflammation. The effect of ginger extract on reducing COX-2 gene expression in colon cancer HT-29 cells was investigated in this study.

Methodology: In this laboratory research, HT-29 cells of colon cancer were cultured in a 75 ml flask. The cells were trypsinated at the third passage and 5000 of them were cultured in the wells of 96-well plates. Ginger extract at four concentrations (10, 20, 30, and 40 mg/mL) was added to the wells, and the plates were incubated at 37˚C for 24, 48, and 72 hours in a 5% Co2 atmosphere. To study the effects of ginger extract on the cells, the MTT test was performed and the densities of the plates were read using an ELISA instrument. Total cellular RNA was extracted and expression of the COX-2 gene was investigated using the RT-PCR method. A sample not treated with the ginger extract was used as the positive control and GAPDH as the internal control.

Results: The measured IC50 concentration of the ginger extract was 20 mg/ml. Determination of the expression of the COX-2 gene using the RT-PCR method indicated that the ginger extract at 20 mg/ml reduced expression of the COX-2 gene compared to using Aspirin. Therefore, ginger extract could be used as an anti-inflammatory drug against the HT-29 cells of colon cancer. 

 

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CONCEPTUAL SUBSTANTIATION OF PRIORITY CRITERIA OF QUALITY OF LIFE AND HEALTH

Conceptual substantiation of priority criteria of quality of life and health in assessing the effectiveness of health care, social and environmental programs

 

Luchkevich V.S., Shakirov  A.M., Marinicheva G.N., Samodova I.L.,

                              Russia, Saint-Petersburg

 

International University of Fundamental Science

 

International Academy of Ecology and Human and Natural Security

 

North-Western Public Medical I. I.  Mechnikov University

 

Factors of health and disease risk existing in the world civilization, problems of ecological safety and unmet subsistence needs –they are on one side, and measures of active preventive health conservation, increase in life expectancy and optimal living conditions – are on another shore.A connecting bridge, ensuring the unity and the ability to implement socially -oriented state policy priority of health and ideological activities, protection of the rights and well-being, is to optimize the quality of life of the population as the main criterion for the effectiveness of social and environmental programs .

 

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PREHENSIVE HEALTHY NOOSPHERIC TECHNOLOGIES

COMPREHENSIVE HEALTHY NOOSPHERIC TECHNOLOGIES

 

PhD in energo-informational technologies, the correspondent participant of the Internatonal Academy  of Ecology and Life Safety -

Dr. Sergey Yurievich Tulsky (PhD).

 

    In 1990 the author put forward a hypothesis that all the biological forms of life in total form are some kinds of a rational substance in the form of energo-informational field. Such a hypothesis emerged on the basis of the analysis of the reasonable behavior of birds in flock, bees, ants, locusts, fish and other biological poulation.  This hypothesis corresponded to the doctrine by V.I.Vernadsky about the transition of biosphere into noosphere, that is reasonable sphere. There was the opinion that all the people living on the planet and that is 7, 5 billion people, in total, form the united energo-informational field that has reasonable characteristics.  Such field according to the author’s opinion has to exist according to the law of transition from quantity to quality and the law of conservation of energy. If we turn to Pierre Teilhard de Chardin, co-author of the term “noosphere”, then his term is put down to the collective consciousness. The hypothesis now has to be approved or disapproved.  Due to the biolocation, the means of connection to the different objects on the big distances, the attempt of connection to the supposed egergoinformational field was made.  This attempt turned to be successful. The dialog with energo-informational field took place, that is, Noosphere. In the result of the dialog the information in the form of knowledge about the appearance of biological life forms on the planet was gained, also about the mechanisms of matter motion and the harmonious human development. The author called the knowledge gained in this way: “Noospheric studies” The main postulate of the noospheric studies sounds: “All the processes in the Universe, evident or latent” happen due to the information on the basis of what the matter is constructed, and it moves.

 

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ABOUT THE UNCERTAIN CONSEQUENCES OF NUCLERA EXPLOSIONS IMPACT ON THE BIOSPERE OF THE EARTH

ABOUT THE UNCERTAIN CONSEQUENCES OF NUCLERA EXPLOSIONS IMPACT ON THE BIOSPERE OF THE EARTH

Grand Ph. D. V.A.Shemchyk

Today eight nuclear powers have in total nuclear weapons in trinitrotoluol equivalent 320 thousand of megatons in store. If,  in case of war between Europe, Russia, the USA, at least 10 % of these recourses were used, after a month of the start of the war 1300 million of the world population of 1400 would die. From the rest 100 million 70 million would be seriously wounded, and 30 million slightly injured people would be obliged to solve the problems, which occurred because of the total destruction of the civilization.

 

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STUDY INVESTIGATING PREDICTORS INFLUENCING LACK OF WESTERN PSYCHIATRIC TREATMENT SEEKING BEHAVIOR FOR DISSOCIATIVE TRANCE

By Dr. Gamini Jayasinghe,MBBS, MSc,  PhD Reading

Introduction and classification-

Dissociation has been described in psychiatric literature as early as 1815. Before the publication of the fourth edition (DSM-IV) of diagnostic and statistical manual of mental disorders in 1994, the condition was known as multiple personality disorder. The name was changed to address both misconceptions regarding the nature of the disorder and the clinical transition from a rare to a more commonplace diagnosis. Pathological possession trance (PPT) was formally known as dissociative trance disorder in the DSM psychiatric manual and currently PPT is included within the dissociative identity disorder (DID) criteria in the DSM-IV. Psychiatric diagnosis of DID has been under serious scrutiny for more than 10 years. Dissociative identity disorder( DID) is the most complex of dissociative identity disorder (American psychiatric association,1994 ).

Possession and trance disorder or pathological possession trance is characterized by a transient alteration in identity and one’s normal identity is temporarily replaced or possessed by a spirit, ghost, deity, or other person. This condition is    associated with stereotype involuntary movements or amnesia. Why I interest for this disorder is one of the most common dissociative disorders in Asia. The important fact is PPT covers only the involuntary possession experience.

 

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TRADITIONAL AND AYURVEDA MEDICINE IN SRI LANKA

Dr. Hettige M., PhD (A.M.) B.A.M.S.(Hon)AY M. Ac. F

 Traditional medicinal  Treatments in Sri Lanka dates back to the pre Aryan civilization and it goes back as far as the 3rd century before Christ .The earliest systems of Medicines which functioned before the introduction of Ayurveda was the indigenous systems of Medicine in Sri Lanka. This could be proved by taking into consideration the archeological inscriptions, Ola leaf manuscripts and the ancient historical writing namely Mahawansa and Chulawansa in Sri Lanka. However the archeological findings reveal that the standards of health services maintained in the ancient past are highly commendable, and were in keeping with modern concept of primary health care, which illustrates instances and examples of treatments even before Buddhism was introduced to Sri Lanka by Rev. Mahinda son of great king Asoka of India. Sri Lankans had practiced Traditional Medicines and thereafter with the introduction of Ayurveda Medicines, it got mixed up with our traditional Medicinal systems and they are practiced very widely today. However the Ayurveda treatments have not forgotten the traditional medical systems and they are embedded in Ayurveda.

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