Haghighi, A.1, Zia Jahromi, N.2, Hejazi, S.H.3
1- MSc. In Biochemistry, School of Basic Sciences, Islamic Azad University/Shahrekord Branch
2- Assistant Professor in Biology group, School of Basic Sciences, Islamic Azad University/Shahrekord Branch
3- Professor in the Sedigheh Tahereh Skin Diseases and Leishmaniasis Center, Parasitology and Mycology Group, School of Medicine, Esfahan University of Medical Sciences
Introduction and objective:Colon cancer is the second reason of mortality caused by cancer in the world, after lung cancer, and it is the third prevalent gastrointestinal cancer after gastric and esophageal cancers in Iran. Environmental and genetic factors have important roles in the emergence of this cancer. One of the important factors in creation of this type of cancer is chronic inflammations of colon. Ginger has anti-inflammatory properties due to its content of -gingerol and, hence, it can play a role in the prevention of colon cancer. COX-2 gene expression is increased in colon cancer, causing intestinal inflammation. The effect of ginger extract on reducing COX-2 gene expression in colon cancer HT-29 cells was investigated in this study.
Methodology: In this laboratory research, HT-29 cells of colon cancer were cultured in a 75 ml flask. The cells were trypsinated at the third passage and 5000 of them were cultured in the wells of 96-well plates. Ginger extract at four concentrations (10, 20, 30, and 40 mg/mL) was added to the wells, and the plates were incubated at 37˚C for 24, 48, and 72 hours in a 5% Co2 atmosphere. To study the effects of ginger extract on the cells, the MTT test was performed and the densities of the plates were read using an ELISA instrument. Total cellular RNA was extracted and expression of the COX-2 gene was investigated using the RT-PCR method. A sample not treated with the ginger extract was used as the positive control and GAPDH as the internal control.
Results: The measured IC50 concentration of the ginger extract was 20 mg/ml. Determination of the expression of the COX-2 gene using the RT-PCR method indicated that the ginger extract at 20 mg/ml reduced expression of the COX-2 gene compared to using Aspirin. Therefore, ginger extract could be used as an anti-inflammatory drug against the HT-29 cells of colon cancer.